Abstract:
In the poultry sector, significant financial losses are attributed to veterinary infections known as salmonellosis. Salmonella ranks among the leading foodborne pathogens impacting global food producers and public health systems. The indiscriminate use of antibiotics has contributed to the proliferation of multidrug-resistant bacterial infections in livestock, diminishing the effectiveness of traditional treatment methods for infectious diseases. This situation has sparked renewed interest in phages among researchers since the early 21st century. Phages are abundant in nature, with up to 1031 particles present. In this study, we have successfully isolated and analyzed the Sal_J1phage, which targets Salmonella Typhimurium, a primary cause of Salmonellosis. We assessed various characteristics of the Sal_J1 phage, including its stability under different heat and pH conditions, its one-step growth curve, host range, and how cations influence its adsorption rate to host species. The Sal_J1 phage produced well-defined boundaries and clear plaques, measuring between 1.7 and 3.2 mm, and exhibited a narrow host range, affecting only a few isolates of the bacteria under investigation. The phage could endure pH levels from 4 to 10 and withstand a temperature of 70°C for 60 minutes.Sal_J1 demonstrated an optimal multiplicity of infection ranging from 0.1 to 1.Transmission Electron Microscopy identified Sal_J1 as part of the Myoviridae family, featuring a contractile tail 25 nm wide and 140 nm long, with a 55 nm head. The one-step growth kinetics indicated latent periods of less than 22minutes and a burst size of 256 PFU/ Virion. These characterizations of theSal_J1 phage provide a foundation for selecting the most effective bacteriophage to manage bacterial infections in the poultry industry.
Page(s):
95-95
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
poultry
,
Resistance
,
Salmonella typhimurium
,
Phage therapy