An enzyme-linked immunosorbent assay for brucella specific antibody and real-time PCR for detecting brucella spp. in milk and cheese in sanliurfa, turkey. | [Pakistan Veterinary Journal • 2017]

Author(s):

1. Serap Kiliç Altun: Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Harran, Sanliurfa, Turkey

2. Akin Yigin: Department of Genetic, Faculty of Veterinary Medicine, University of Harran, Sanliurfa, Turkey

3. Sevil Erdenlig Gürbilek: Department of Microbiology, Faculty of Veterinary Medicine, University of Harran, Sanliurfa – Turkey

4. Semra Gürbüz: Higher School of Tourism and Hotel Management, University of Mardin Artuklu, Gastronomy and Culinary Art, Mardin– Turkey

5. Mehmet Demirci: Cerrahpasa Medical Faculty, Medical Microbiology, University of Istanbul, Istanbul–Turkey

6. Oktay Keskin: Department of Microbiology, Faculty of Veterinary Medicine, University of Harran, Sanliurfa – Turkey

7. Osman Yasar Tel: Department of Microbiology, Faculty of Veterinary Medicine, University of Harran, Sanliurfa – Turkey

Abstract:

The objective of this study was to investigate the presence of anti-Brucella antibody and Brucella spp. DNA in cow, sheep and goat milk and in Urfa cheese collected from markets and bazaars in Sanliurfa, located in southeast of Turkey. A total of 258 samples consisting of 178 raw milk (48 cow milk, 65 sheep milk and 65 goat milk) samples and 80 Urfa cheese samples were investigated. AntiBrucella antibody was detected by indirect ELISA (i-ELISA), and the presence of Brucella spp. DNA was screened by real time Polymerase Chain Reaction (RTPCR). 16.6% of the cow, 6.1% of the goat and 6.1% of the sheep milk and 16.25% of the cheese samples were found as positive for brucella antibodies by iELISA. The RT-PCR assay amplified Brucella DNA from 18.75, 7.6 and 6.1% cow, goat and sheep milk samples respectively. Brucella DNA was amplified from 22.5% cheese samples. The 11.2% and 13.9% of the samples were found as positive by i-ELISA and RT-PCR respectively. This study indicates that milk and milk products consumed in Sanliurfa poses a risk to public health in terms of brucellosis. The combining usage of both i-ELISA and RT-PCR methods could lead to more reliable results to detect anti-Brucella antibody and Brucella spp. DNA from milk and cheese samples.

Page(s): 39-42

DOI: DOI not available

Published: Journal: Pakistan Veterinary Journal, Volume: 37, Issue: 1, Year: 2017

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