Abstract:
Heat stress - a big issue of 21st century; is one of the major abiotic stresses that effect plant growth and productivity. Upregulation of expression of heat shock proteins and heat shock factors upon heat stress is an obvious phenomenon occurring in plants. These chaperones have also been implicated in diverse biological processes andplay a key role in signal transduction and gene regulation under stress condition. To evaluate the differential expression of heat shock proteins and heat shock factors in tomato F1 hybrids an experiment was conducted in Arid Agriculture University, Rawalpindi in 2021. The selected genotypes of tomato (10145, 17903 and SAMRUDHI) were crossed and F1 seedlings and their parents were sown to analyze for their expression of selected HSP and HSF genes at five-week-old seedling stage. Reverse transcriptase polymerase chain reaction was used to investigate the differential expression of HSP100, HSP90.1, HSP90.2, HSP70.1, HSP70.2, HSP 60, HSP40, HSP26.5, HSFA1a, HSFA1b, HSFA2, HSFA3, HSFB1, HSFB2a, HSFB2b, HSFA7 and HSFA8. Variation in differential response of HSP and HSF genes in the F1 hybrid and the parents upon heat stress has detected. The most obvious upregulation was pointed out for F1 hybrid i.e., SAMRUDHI ×17903 under heat stress treatment at 45°C. The upregulation in expression levels of HSPs and HSFs group of genes suggested that specific patterns of F1 hybrid was involved in SAMRUDHI ×17903 under heat stress. This may be due to new regulatory interactions of genes of the two parents involved in the hybrid development.
Page(s):
246-246
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Tomato
,
heat stress
,
heat shock proteins
,
Heat shock factors
,
F1 hybrids