Abstract:
A pot experiment was carried out at the Agronomy shade house, Department of Agronomy, Hajee Mohammad Danesh Science and Technology University, Dinajpur -5200 from December 2023 through June 2024 to determine the role and application of PGPR in combating the salt stress of wheat (BWMRI Gom 1). The experiment comprised five treatments viz. i) S0B0 (0 mM NaCl + 0 PGPR) ii), S150B0 (150 mM NaCl + 0 PGPR), iii) S150B1 (150 mM NaCl + PGPR- Serratia sp. Strain HSTU-BF1R2), iv) S150B2 (150 mM NaCl + PGPRSerratia sp. Strain HSTU-BF3R4), v) S150B1+B2 (150 mM NaCl + Serratia sp. Strain HSTU-BF1R2+ Serratia sp. Strain HSTU-BF3R4). The experiment was laid out in Completely Randomized Design (CRD) with three replications. Salinity stress markedly reduced wheat growth, physiological traits, yield components, and nutrient status, whereas PGPEB application effectively alleviated these adverse effects. The combined inoculation (S150B1+B2) consistently produced the highest values across morphological parameters, including plant height (51.73 and 55.59 cm at 60 DAS and FH), number of leaves (6.00 and 8.67), flag leaf area (1026.0 mm²), flag leaf length (140.07 mm), width (8.75 mm), ratio (15.17), fresh and dry leaf weights (1.88 g and 0.48 g), fresh and dry culm weights (2.05 g and 0.38 g), root length (7.48 cm), root fresh weight (0.28 g), and root dry weight (0.18 g). Photosynthetic pigments (Chl a, Chl b, total Chl, carotenoid) and water status (RWC, WRC, CMS) were also highest under S150B1+B2, while canopy temperature was lowest. Yield attributes improved significantly, with maximum spike length (13.11 cm), spike weight (0.50 g), grains spike?¹ (13.00), 1000-grain weight (3.18 g), grain yield (0.35 g plant?¹), biological yield (0.61 g plant?¹), and harvest index (51.01%). Nutrient analysis revealed the highest K (30.62 mg g?¹ DW), Ca (0.80 mg g?¹ DW), and protein (11.90 mg g?¹ DW) in the dual-inoculated plants. Antioxidant enzyme activity was also significantly affected by treatments. Under control conditions, CAT (0.49), POD (0.21), and APX (0.09 U mg?¹ protein) were lowest, while salinity stress alone (S150B0) caused the highest CAT (1.05), POD (0.65), and APX (0.17), indicating oxidative stress. Inoculation with PGPEB under salinity reduced enzyme activities, with the lowest CAT (0.52), POD (0.11), and APX (0.11) recorded in S150B1+B2, followed by single-strain treatments. These findings demonstrate that PGPEB, particularly the dual inoculation (S150B1+B2), not only enhanced growth, physiological traits, yield performance, and nutrient accumulation but also mitigated oxidative stress by regulating ROS levels, thereby improving the overall resilience of wheat under salinity stress.
Page(s):
34-35
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
yield
,
Growth
,
Physiological traits
,
antioxidants enzymes
,
Salt stress
,
Nutrient status
,
Serratia sp HSTUBF1R2 and HSTUBF3R4
,
Plant growthpromoting endophytic bacteria PGPEB
,
wheat cv BWMRI Gom 1