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A novel Neutral Endoglucanase from Streptomyces Sp. H31 with Wide- Ph-range Stability.
Author(s):
1. Wei-Zhao Chen: College of Life Science, South China Agricultural University, Guangzhou 510642, P.R. China: Shenzhen Key Laboratory for Microbial Gene Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, P.R. China
2. Xiao Yang Ou: Shenzhen Key Laboratory for Microbial Gene Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, P.R. China
3. Sen-Lin Liu: Shenzhen Key Laboratory for Microbial Gene Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, P.R. China
4. Miao Xing: College of Life Science, South China Agricultural University, Guangzhou 510642, P.R. China
Abstract:
An endoglucanase-producing strain Streptomyces sp. H31 was isolated from mangrove soil in Shenzhen, P.R.China, and the endoglucanase gene egH31 was cloned. The open reading frame (ORF) of this gene is 762bp and it encodes 253 amino acid residues, which showed the highest similarity (84%) with the endoglucanase from Streptomyces davawensis. The endoglucanase gene egH31 was successfully expressed in E. coli. We purified the recombinant EgH31 to homogeneity by affinity chromatography. Molecular mass of the recombinant EgH31 is about 31 kDa. The maximum activity of EG-H31 was determined at pH 7.0 and 45°C and shown high stability within a wide pH range (3.0-11.0). EgH31 had good resistibility to SDS, EDTA, EGTA and most of metal ions examined. Most interestingly, EgH31 was stable in commercial laundry detergents, indicating that this enzyme is useful in textile and detergent industry.
Page(s): 893-898
Published: Journal: International Journal of Agriculture and Biology, Volume: 19, Issue: 4, Year: 2017
Keywords:
Keywords are not available for this article.
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