UP-PCR analysis of the seedborne pathogen Fusarium fujikuroi causing bakanae disease in rice | [International Journal of Agriculture and Biology • 2011]

Author(s):

1. Christian Joseph R. Cumagun: Crop Protection Cluster, University of the Philippines Los Baños (UPLB) College, Laguna 4031, Philippines

2. Erwin Arcillas: Crop Protection Cluster, University of the Philippines Los Baños (UPLB) College, Laguna 4031, Philippines

3. Evelyn Gergon: Crop Protection Division, Philippine Rice Research Institute, (PhilRice) Maligaya, Science City of Munoz, Nueva Ecija 3120, Philippines

Abstract:

Twenty isolates of Fusarium fujikuroi from Nueva Ecija and Laguna provinces in the Philippines were characterized by Universally Primed Polymerase Chain Reaction (UP-PCR) using AA2M2 and L45 primer. Sixty-six unique banding patterns were generated and a dendrogram was created. High degree of variation was observed among the isolates. At 90% similarity, fifteen clusters were formed, suggesting that sexual reproduction plays a significant role in generating variation of F. fujikuroi populations in the field.

Page(s): 1029-1032

DOI: DOI not available

Published: Journal: International Journal of Agriculture and Biology, Volume: 13, Issue: 6, Year: 2011

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