An Efficient Method for Direct Shoot Regeneration from Leaf Explants of Solanum nigrum Induced by Thidiazuron. | [International Journal of Agriculture and Biology • 2017]

Author(s):

1. Humera Afrasiab: Department of Botany, University of the Punjab, Lahore-54590, Pakistan

2. Nadia Rashid: Department of Botany, University of the Punjab, Lahore-54590, Pakistan

3. Muhammad Akram: Department of Botany, University of the Punjab, Lahore-54590, Pakistan

Abstract:

Direct shoot regeneration was established from leaf, petiole and nodal explants from in vitro and field grown plants of Solanum nigrum on Murashige and Skoog (MS), and Gamborg's (B5) media containing different levels of plant growth regulator, Thidiazuron (TDZ). Comparison of the individual effect of basal media, levels of TDZ, explant type or source of explants on shoot regeneration was tested. Percent shoot regeneration from leaf explant was highest (86.66%) followed by in vitro source of explant (83.65%), B5 basal medium (83.20%) or 2.27 µM TDZ (73.70%) after 60 days of initial culture. The number of shoots was highest (35.10) with 9.13 cm longest shoot at 2.27 µM TDZ. There was a little correlation between predictors and dependent variables was found; whereas, GLM analysis demonstrated significant correlation between the factors. In vitro shoots were transferred to a half-strength MS medium containing different concentrations of NAA or IBA in MS or B5 medium. MS medium produced 100% roots supplemented with 0.5 µM NAA after 28 days. However, stout and hardy rooted shoots were obtained at 0.5 µ IBA with 10.19 mean number and 5.57 cm long roots. Rooted shoots were shifted to ex vitro conditions in polyethylene-cups filled with peat moss: sand: garden soil (1:1:1) with 85.50% survival with true-totype morphology under the natural conditions. The present investigation demonstrated that TDZ may be used for high frequency direct shoot regeneration from leaf explants of S. nigrum on B5 basal medium. This protocol may be useful for subsequent high yielding metabolites producing plants.

Page(s): 348-354

DOI: 10.17957/IJAB/15.0288

Published: Journal: International Journal of Agriculture and Biology, Volume: 19, Issue: 2, Year: 2017

Keywords:

Keywords are not available for this article.

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[41] 2017.,(Received 26 October 2016; Accepted 04 February -

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