Abstract:
Keeping in view the pharmacological importance, the extraction of leaves of Alstonia scholaris Linn and Plumeria rubra was performed in methanol, ethyl acetate and water solvents. The well-diffusion method experiment along with 1,1-diphenyl-2-pycrilhydrazil (DPPH) free radical scavenging assay were used to assess the antimicrobial and antioxidant activity, respectively. The assays of alphaamylase inhibition and antiglycation were used to evaluate the antidiabetic effect. Fourier transform infrared spectroscopy (FTIR) and high-performance liquid chromatography (HPLC) contributed in the identification of functional groups and compounds identification respectively. The methanolic extract of both Alstonia scholaris Linn and Plumeria rubra showed the highest antimicrobial potential against both E. coli and S. aureus possessing the highest zone of inhibitions among all samples. The MIC evaluation of methanolic extracts of plants was done by colorimetric analysis which resulted as 5mg/mL for Alstonia scholaris Linn and Plumeria rubra. While determining the antioxidant profile, the aqueous extract of Alstonia scholaris showed the highest total phenolic contents (586.78±0.364mg gallic acid equivalent / 100 g dry weight) while the highest flavonoid content (807.36±0.66mg catechin equivalent/ 100g dry weight) was possessed by methanolic extract of the same plant. In radical scavenging capacity, it was Plumeria rubathat surpassed other extracts. The methanolic extract of P. ruba showed the highest antiglycation activity (70.32%) while methanolic extract of Alstonia scholaris showed the highest percentage of alpha-amylase inhibition (61.19%). FTIR confirmed the presence of various functional groups such as alcohols, phenols, carboxylic acids, alkanes and aldehydes. The HPLC analysis led to the detection of various phytochemicals including quercetin, gallic acid, vanillic acid, benzoic acid, chlorogenic acid, syringic acid, sinapic acid, chlorogenic acid, M-Coumaric acid and ferulic acid. Both plants demonstrated pharmaceutical potential, which may be useful in the management of diabetes mellitus.
Page(s):
351-351
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Antioxidant activity
,
antimicrobial activity
,
Phytochemicals
,
Antiglycation activity
,
Methanolicextracts