Acid phosphatase: a possible marker of callus senescence and necrosis in chickpea (Cicer arietinum L.). | [Pak phyton • 1989]

Author(s):

1. J. Iqbal: Botany Department, University of Punjab, Quaid-e- Azam Campus, Lahore, Pakistan

2. M. Haroon: Botany Department, University of Punjab, Quaid-e- Azam Campus, Lahore, Pakistan

3. M. S. Ahmad: Nuclear Institute for Agriculture & Biology (NIAB), Faisalabad, Pakistan

Abstract:

Callus formation was obtained from whole seeds of chickpea on MS medium supplemented with 2 mg/L 2, 4-D, I mg/L BA and 0.5 mg/L NM. Seed callus was subcultured upto 5th passage. The calli from different subcultures showed clear differences in their proliferation rate (weight basis), colour and texture. The acid phosphatase activity decreased from main callus to 3rd subculture calli. Thereafter a rapid rise in enzyme activity was observed in the 4th subculture and further rise in the fifth subculture calli. The increase in enzyme activity seems to be a consequence of senescence process. The changes in the specific activity of the enzyme are correlated with callus senescence and browning.

Page(s): 119-125

DOI: DOI not available

Published: Journal: Pak phyton, Volume: 3, Issue: , Year: 1989

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