Abstract:
Fritillaria cirrhosa D. Don syn. F. roylei of Lillaceae family is primarily found in temperate regions of Northern hemisphere. In South Asia, it grows in Himalayan sub-alpine in range of 2800-4000m and is commonly known as 'parrhipayaz' or 'Ban payazi'. F. cirrhosa has anti-asthmatic, anti-microbial, anti-ulcer, anti-tussive, febrifuge, anti-viral, anti-hypertensive, haemostatic and skin soothing properties. F. cirrhosa is one of the eighteen species that are traded actively and demand is increasing while supply is rapidly declining. It is declared as critically endangered because of over-exploitation and unfavorable biotic and abiotic pressure, so its collection is banned in India, Nepal and China. Moreover, unfavorable biotic and abiotic conditions along with slow germination, low rate of viable bulbs growth and narrow growing season also reducing its natural populations. In Azad Jammu and Kashmir (AJK), its market value is 20,000-30,000 PKRs/kg. So, there is need to develop in vitro culturing protocol to help in future conservation trials in Pakistan. In the present work, among all the explants, bulb showed the highest callus response (80%) on MS media at 1.5mg/L BAP+0.4mg/L 2,4 D followed by leaf (50%) at 0.5mg/L BAP+0.5mg/L NAA and then stem (40%) at 1.5 mg/L BAP and 0.4 mg/L 2,4-D. MS media fortified with 2mg/L BAP and 0.5mg/L 2,4-D showed high regeneration from bulb and leaf explants. Bulb formation was observed to be enhanced at 1 mg/L BAP and 1 mg/L NAA. Plants shifted into potted soil under shady place showed same growth to field grown plant. This tissue culture developed system can be used for restoration of natural population from in vitro generated plantlets with improved or high regeneration rates.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
Leaf
,
Regeneration
,
Callogenesis
,
Stem explan
,
Bulb
,
in vitro culture
,
F cirrhosa tissue culture