Use of conductivity measurements for the determination of growth rate of Solanum tuberosum L. cell cultures. | [Journal of Engineering and Applied Sciences • 1998]

Author(s):

1. Muhammad Akbar Anjum: University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan

2. Zafar Mahmud: Statistics Department, Bahauddin Zakariya University, Multan, Pakistan

Abstract:

The suitability of medium conductivity measurements for determining growth in plant cell culture was tested using the suspension cultures of potato (Solanum tuberosum L.) cvs. Desiree and Maris Piper. A perfect positive relationship between packed cell volume and cell number was found during the growth of cells in suspension culture. A perfect negative correlation between increase in packed cell volume and decrease in medium conductivity was observed from the experiment. The measurement of cell concentration by cell counting also showed a negative correlation with the conductivity of culture medium in both cultivars tested. Regression analysis was used to know the rate change in the dependent variable subject to a unit change in explanatory variable.

Page(s): 13-18

DOI: DOI not available

Published: Journal: Journal of Engineering and Applied Sciences, Volume: 17, Issue: 1, Year: 1998

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