THE MOSQUITO MICROBIOME: ANALYSIS FOR ENDEMIC AND EMERGING PATHOGENS OF PUBLIC HEALTH SIGNIFICANCE IN DISTRICT FAISALABAD, PAKISTAN | [Pakistan Journal of Agricultural Sciences • 2021]

Author(s):

1. Iqra Yousaf: Department of Zoology, Government College University,Faisalabad,Pakistan

2. Shabab Nasir: Department of Zoology, Government College University,Faisalabad,Pakistan

3. Syed Makhdoom Hussain: Department of Zoology, Government College University,Faisalabad,Pakistan

4. Azhar Rafique: Department of Zoology, Government College University,Faisalabad,Pakistan

5. Muhammad Umar Ijaz: Department of Zoology, Wildlife and Fisheries, University of Agriculture,Faisalabad,Pakistan

6. Muhammad Asrar: Department of Zoology, Government College University,Faisalabad,Pakistan

7. Saif-Ur-Rehman: Department of Entomology, Northwest A&F University,Yangling, Shaanxi,712100,P.R. China

8. Bilal Ahmad: Department of Zoology, Government College University,Faisalabad,Pakistan

9. Naveed Ahmad Khan: Institute of Pure and Applied Biology, Zoology division, Burhanuddin Zakariya University,Multan,Pakistan

10. Mahid Iftikhar: Department of Zoology, Government College University,Faisalabad,Pakistan

Abstract:

Pakistan being a tropical country, characterized by poor socioeconomic conditions, is more vulnerable to Mosquito-borne diseases. So, this study was proposed to know the prevalence of mosquito-borne diseases in Faisalabad district, Pakistan. For this, mosquito adults and larvae were collected from different habitats with aspirator and dippers respectively from eight towns of Faisalabad district. These samples were carried alive to laboratory and identified with the help of identification keys. Aedes female mosquitoes were killed and stored at -20°C for RNA extraction. Slides were prepared from blood of female Anopheles mosquitoes for the identification of plasmodium under microscope. The genome RNA from female Aedes mosquitoes were extracted as per commercially available kits according to manufacturer guidelines and stored at -40 0C for further use. Using online software, primers and probes for the known human pathogens were designed in consensus with the help of available genome database for different pathogens such as dengue virus. Plasmodium was detected from the mosquitoes that were mostly collected from rural areas and this was generally detected from the samples collected from Jaranwala and Samundri town. Dengue virus was detected generally from city areas and serotype 2 and 3 was detected from the Aedes mosquitoes. Dengue virus was mostly detected from Lyallpur, Madina and Jinnah towns. Mosquitoes were mostly collected after monsoon season in the months of August-September and in spring (March). The disease pathogens were also detected during these months. Through the surveillance of pathogens, we can focus our attention on the hot spots and apply control measures to control mosquitoes for better management.

Page(s): 161-167

DOI: 10.21162/PAKJAS/21.9854

Published: Journal: Pakistan Journal of Agricultural Sciences, Volume: 58, Issue: 1, Year: 2021

Keywords:

rural areas , malaria , Faisalabad , Mosquitoes , serotypes of dengue fever

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