Abstract:
Quinoa (Chenopodium quinoa Willd.), family Chenopodiaceae, is a newly introduced crop in Pakistan. Various Chenopodium spp. growing as weeds in Pakistan possess antifungal potential against Macrophomina phaseolina, the cause of root diseases in hundreds species of plants. This study was done to check antifungal potential of quinoa leaves against this notorious soil-borne pathogen. The pathogen was isolated from diseased mungbean plants and identified on the basis of morphological characters and further confirmed using universal and specific primers namely ITS, ß-tubulin, GADPH and MPRT. Methanolic leaf extracts (1, 2, 3, 4 and 5%) of four quinoa varieties namely V1, V2, V7 and V9 were evaluated for their antifungal activity. Various extracts suppressed fungal biomass by 36-87%. Extract of V7 exhibited the best activity, reduced fungal biomass by 73-87% and was therefore selected for further bioassays. This extract was fractionated using three organic solvents of variable polarities namely n-hexane, chloroform and ethyl acetate. Antifungal bioassays were conducted using 8 concentrations (1.562 to 200 mg mL-1) of each fraction. All the concentrations of chloroform fraction completely controlled (100%) growth of the pathogen. n-Hexane and ethyl fractions were comparatively less inhibitory and caused 32-100% and 35-100% reduction in fungal biomass, respectively. GCMS examination of chloroform fraction indicated that 1,2-benzedicarboxylic acid, diisooctyl ester was in the highest abundance with peak area of 29.83% followed by acetic acid,2-(2,2,6-trimethyl-7-oxa-bicyclo[4.1.0] hept-l-yl)-propenyl ester (6.75%) and 9,12-octadecadienoic acid(Z,Z)- (6.46%). It concludes that chloroform fraction of leaf extract of V7 possess potent antifungal compounds against M. phaseolina.
Page(s):
6-6
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Sustainable Agriculture and Food Security, August 27-31, 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
quinoa
,
leaf extract
,
Macrophomina phaseolina
,
Chloroform fraction
,
Antifungal constituents