A novel point mutation in rpsL gene of Septomyces coelicolor with enhanced blue pigment production. | [Pakistan Journal of Biotechnology • 2006]

Author(s):

1. Hechun Zhang: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

2. Wu Haizhen: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

3. Huizhan Zhang: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

4. Yuanxing Zhang: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

Abstract:

Three strains showing enhanced blue pigment production were screened among thirty streptomycin resistant mutants of Streptomyces coelicolor 100. Their yields of blue pigment were all improved, especially for mutant S-3. Analysis of the mutants revealed that a point mutation occurred in mutant S-3. In rpsL gene, A to G transition at the position 128 resulted in Lys-43 to Arg substitution in the ribosomal protein S12 encoded by rpsL gene. There was no mutation in rpsL gene of other two streptomycin resistant mutants. Compared with the wild type strain, the mutant S-3 showed increased blue pigment yield and slightly decreased growth rate. This strategy of streptomycin resistance induction could be especially effective for improving the blue pigment production from the wild type strain of Streptomyces coelicolor.

Page(s): 93-97

DOI: DOI not available

Published: Journal: Pakistan Journal of Biotechnology, Volume: 3, Issue: 1--2, Year: 2006

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