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Unveiling the pharological applications of biosynthesized silver and nickle oxide nanopaticles using lepidium sativum mucilage polysaccharides
Author(s):
1. Afghana Firdous: Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
2. Urooj Begum: Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
3. Aqsa: Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
4. Amjad Iqbal: Department of Food Science & Technology, Abdul Wali Khan University Mardan, Pakistan
5. Ayaz Ahmad: Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
Abstract:
Nanotechnology, a rapidly evolving field, focuses on the synthesis, manipulation, and application of nanoscale materials. In recent years, bio-assisted synthesis has gained considerable attention as a means to overcome the limitations associated with conventional physical and chemical methods. This study aimed to synthesize silver (Ag) and nickle oxide (NiO) nanoparticles (NPs) using the watersoluble polysaccharides extracted from Lepidium sativum (cress) seed mucilage. Mucilage was extracted by soaking cress seed while polysaccharides were precipitated by treating mucilage with 75% ethanol for overnight. The biosynthesized AgNPs and NiONps were confirmed through spectroscopic techniques. The UV visible spectrum of the AgNPs gives a sharp absorption peak at 448nm while that of NiONPs was recorded at 343nm. The results revealed that AgNPS was able to inhibit the growth of five human pathogenic Staphylococcus aureus (4.5mm ± 0.67mm), Escherichia coli (4.12mm ± 1.09mm), Klebsiella pneumonia (3.67mm ± 0.19mm), Salmonella typhi (5.16mm ± 1.23mm) and Shigella (2.9mm ± 0.85mm). AgNPs and NiONPs also exhibited significant antioxidant activities by scavenging the DPPH free radical having with the EC50 value 67.53 and 109.68µg/mL, respectively. These results suggest that water-soluble polysaccharides from cress seed mucilage has the ability to synthesized AgNPs and NiONPs with strong antimicrobial and antioxidant potential and could be exploited for further biomedical applications. This study concludes that utilizing mucilage polysaccharides
Page(s): 107-107
DOI: DOI not available
Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Nanoparticles , Antioxidant , Polysaccharides , Lepidium sativum
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