Abstract:
Uric acid and its role as a metabolic waste product, uric acid has been proposed to be an important molecule with multiple functions in human physiology and pathophysiology and may be linked to human diseases beyond nephrolithiasis and gout. The kidney is an important regulator of circulating uric acid levels, by reabsorbing around 90% of filtered urate. Defective renal handling of UA is a frequent pathophysiologic factor underpinning hyperuricemia and gout. In the current study, copper dopped hydroxyapatite based biosensor was developed to identify uric acid in blood serum samples from arthritis patients. Different characterization techniques, were used such as FTIR, SEM, TGA, EDX and XRD, were used to characterize the prepared nanocomposite. In the presence of TMB H2O2 can catalyze the endogenous per oxidase-like behavior of the copper doped hydroxyapatite nanocomposite. Based on this phenomenon, copper doped hydroxypatite-based colorimetric sensor was prepared for the detection of uric acid. To achieve the best possible results, various optimized parameter were optimized such as the amount dopping, H2O2, pH, TMB, and uric acid. With a linear range from2 x 10-9 to 3.22 x 10-7 M, a low detection limit of 1.3 x 10-8 M, a low quantification limit of 4.3 x10-8 M, and an R2 of 0.9996, the suggested sensor as sensitive and selective for uric acid detection e. Optimum time for the proposed sensor is 2 minutes at room temperature. The proposed sensor has been successfully applied to arthritis patientsblood serum samples for uric acid detection with a visible colorimetric change.
Page(s):
9-9
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Uric Acid
,
Copper doped Hydroxyapatite
,
colorimetric sensor
,
TMB