Abstract:
Quinoa (Chenopodium quinoa Willd.), family Chenopodiaceae, is gaining importance worldwide due to its use in food and pharmaceutical industry. It is native to Andean regions of South America and is recently introduced in Pakistan. The present study was carried out to assess the activity of inflorescence extract of four quinoa varieties against Macrophomina phaseolina, an opportunistic human pathogen responsible for burns, inflammation, skin trauma and ulcerative lesions. Methanolic inflorescence extract of four quinoa varieties having concentrations 1, 2, 3, 4 and 5% (w/v) were examined in vitro against M. phaseolina. These extracts caused 33-87% inhibition in growth of the pathogen. Variety V7 exhibited the best antifungal potential by arresting the fungal growth from 7687% and thus was selected for further fractionation by using four organic solvent solvents of different polarities. Antifungal screening bioassays were performed with eight concentrations ranging from 1.562 to 200 mg mL-1 of each of these fractions. All the concentrations of chloroform fraction completely retarded the pathogen growth whereas, n-butanol fraction arrested the pathogen growth by 56-100%. GC-MS analysis of chloroform and n-butanol fractions was carried out to explore their phytochemical profiles. Literature survey showed that among the identified compounds, (Z,Z)-9,12octadecadien-1-ol (23.76%); 1-(+)-ascorbic acid, 2,6-dihexadecanoate (11.05%); 8,11octadecadienoic acid, methyl ester (7.11%); hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester (5.40%); D-allose (4.77%); 11-eicosenoic acid, methyl ester (4.08%); and desulphosinigrin (3.11%) are known for their antifungal activity. Therefore, the present investigation concludes that chloroform and n-butanol fractions of quinoa variety V7 inflorescence extract contain potent antifungal compounds effective against M. phaseolina growth.
Page(s):
436-436
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
GCMS
,
quinoa
,
Macrophomina phaseolina
,
Fungicidal Potential