Polyurethanes (PUs) are commonly used chemicals in various industries and are conventionally produced by the reaction of polyols and diisocyanates. However, synthetic petroleum-based polymers are non-biodegradable and resulting in issues like waste disposal, risks to aquatic and terrestrial ecosystems. In this study, a novel microalgal protein-based ultraviolet (UV) curing polyurethane acrylate (PUA) was prepared with hexanediol diacrylate (20% HDDA) precursor, amino acid oligomers, and photoinitiator (Irgacure 184). Experimental conditions for the synthesis of precursors were studied and their chemical structures were confirmed by FT-IR, 1HNMR, 13CNMR, and differential scanning calorimetry (DSC). The absorption peak at 1695 cm-1 confirmed the formation of a urethane bond. For instance, the obtained polyurethane acrylate coating (PUA) possessed a glass transition temperature (Tg) of 122 ?, tensile strength, modulus of 19.1 MPa, 465 MPa, and lower elongation break (6%). While using reactive diluents, the coating compositions showed significant enhancement of mechanical, physical, and chemical resistance properties. In general, this work offered a simple bioscheme of reactions to prepare a sustainable microalgal-based PU coating that could be applied in the coating industry with improved properties.