Author(s):
1. Tooba Khan:
Dept. of Microbiology, University of Karachi, Pakistan; Dept. of Applied Sciences, Faculty of Engineering Sciences and Technology, Hamdard University, Karachi, Pakistan
2. Muhammad Sohail:
Dept. of Microbiology, University of Karachi, Pakistan
Abstract:
Objectives: Arthrocnemum macrostachyumis a halophytic plant that can survive in considerably high salt concentrations; up to 200mM. Endophytic bacteria associated with them are not only capable of enhancing their survival ability in hyper-saline soils but also known to produce various extracellular enzymes. In this study, the multi-enzyme preparation obtained from the isolated endophytes from this plant was first used for the saccharification of its own biomass and thissaccharified biomass was then used as a natural adsorbent to remove methylene blue dye from its aqueous solution. The utilization of natural substrates for dye removal have now become the research focus as they are abundantly available, easily up-graded and high adsorptiom capacities then synthetic adsorbents. Methods: The endophytic bacteria were screened for the production of four extracellular enzymes i.e. xylanase, cellulase, pectinase and amylase by qualitative analysis using 1% specific substrate medium and quantitative analysis using standard DNS method. 50 IU ml-1of the crude multi-enzyme preparation was then added in a flask containing the powdered biomass of A. macrostachyumin 50mM sodium citrate buffer of 3.5 pH and 0.2% sodium azide. Followed by the saccharification, 20 ml of 20 mgL1 of MB solution was added with 0.1% w/vsaccharified biomass and effect of pH and time on the dye adsorption was analyzed. Moreover, the kinetic parameters were obtained by Pseudo-First and Pseudo-Second order models which indicates the efficiency of adsorbent. The release of reducing sugar was found to be 0.66g.g-1 and 0.42 g.g-1of the substrate indicating the effective saccharification of biomass by the crude multi-enzyme extract. Maximum dye adsorption was attained at pH 10 (95%) and after 60 minutes (97.8%). Moreover, a comparative analysis of dye removal with the unsaccharified biomass (76%) as an adsorbent also showed the increased efficiency of saccharified biomass in removing dye. The kinetic parameters revealed that the adsorption process followed Pseudo-second order model with high R2 values (>90%) indicating the chemical nature of the adsorption process. The saccharified biomass of A. macrostachyum can be efficiently used in the removal of methylene blue dye as a natural and cheaper source of adsorbent and its further potential can be explored at industrial level. This saccharification can be effectively done by using the crude multi-enzyme extract of the endophytic bacteria isolated from the A. macrostachyum.
Page(s):
150-150
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Methylene blue
,
Adsorbent
,
multienzyme
,
Arthrocnemum macrostachyum
References:
References are not available for this document.
Citations
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