A new method for the isolation and purification of lawsone from Lawsonia inermis and its ROS inhibitory activity. | [Pakistan Journal of Botany • 2013]

Author(s):

1. Syed Muhammad Ghufran Saeed: Department of Food Science and Technology, University of Karachi, Karachi, 75270, Pakistan

2. Syed Asad Sayeed: Department of Food Science and Technology, University of Karachi, Karachi, 75270, Pakistan

3. Seema Ashraf: Department of Food Science and Technology, University of Karachi, Karachi, 75270, Pakistan

4. Shahina Naz: Department of Food Science and Technology, University of Karachi, Karachi, 75270, Pakistan

5. Rahmanullah Siddiqi: Department of Food Science and Technology, University of Karachi, Karachi, 75270, Pakistan

6. Rashida Ali: Department of Food Science and Technology, University of Karachi, Karachi, 75270, Pakistan; Division of Food Research, HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan; English Biscuit Manufacturers (Pvt.) Limited, Plot 1-4, Sector 23, Industrial Area, Karachi-749 00, Pakistan

7. M. Ahmed Mesaik: Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan

Abstract:

Lawsone a coloring component of the Henna (Lawsonia inermis) leaves was found to bind with proteins. Present study has suggested a new rapid method for the isolation of lawsone from L. inermis through the calcium ion using the Flash and Disc Counter Current Chromatography (DCC). The purified pigment structurally characterized by 2D, TLC and NMR spectroscopy, was found to be active against reactive oxygen species (ROS) by Chemeluminiscence assay using lymphocytes. Purified lawsone showed remarkable inhibitory activity on the oxidative burst response of the whole blood, polymorphonuclear cells (PMNCs), Mononuclear cells (MNCs) with IC50 as 13 ±1.6 μg/ml, 11±2.7 μg/ml. and 10±4.2 μg/ml respectively.

Page(s): 1431-1436

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 45, Issue: 4, Year: 2013

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