Abstract:
Current study was conducted at Institute of Biochemistry and Bio-technology, University of Veterinary and Animal Sciences, Lahore, Pakistan during spring 2019. Meat adulteration is a global concern that directly impacts public health, religious beliefs, and fair trades. In the present study, a rapid, cost-efective, reliable, specific and highly sensitive triplex PCR assay has been developed to ensure meat adulteration in halal meat products. The triplex PCR system was optimized to simultaneously identify the goat/sheep, cow/bufalo and horse/donkey DNAs in a single reaction mixture. The primers were designed and optimized targeting 16 S rRNA gene with fragments of 707-bp, 235-bp and 315-bp in size for goat/sheep, cow/bufalo, and horse/donkey. To assess the reproducibility and reliability of the developed triplex PCR assay, commercially available meat products (n=30) were screened, containing both uncooked (n=15) and cooked (n=15) meat samples. The screening results illustrated that meat adulteration was present in four (10%) out of thirty commercially available meat products. The developed assay can be easily applicable in halal food industries food control authorities and food labs.
Page(s):
153-159
DOI:
DOI not available
Published:
Journal: Journal of Agricultural Research, Volume: 60, Issue: 2, Year: 2022
Keywords:
Pakistan
,
Primer
,
Meat Adulteration
,
denaturation
,
crossreactivity
,
annealing