TGF-β Mediates the Role of P53 and microRNA- Related Signaling Pathways in the Pathogenesis of Diabetic Nephropathy in Rats | [Pakistan Journal of Zoology • 2024]

Author(s):

1. Yonghong Ma: Department of Endocrine Metabolism, Afiliated Hospital of Qinghai University,Xining, Qinghai810000,China

2. Jinlan Ma: Department of Nephrology, Afiliated Hospital of Qinghai University,Xining, Qinghai810000,China

3. Qifu Zhang: Department of Trauma Orthopedics, Afiliated Hospital of Qinghai University,Xining, Qinghai810000,China

4. Huajie Zou: Department of Endocrine Metabolism, Afiliated Hospital of Qinghai University,Xining, Qinghai810000,China

5. Shenghua Ma: Department of Endocrine Metabolism, Afiliated Hospital of Qinghai University,Xining, Qinghai810000,China

Abstract:

The objective of this study was to study the relationship between TGF-ß-mediated P53 and microRNArelated signaling pathways and the occurrence and development of diabetic nephropathy. The sample of rats was randomly assigned to a control group and an experimental group, and a rat model of diabetic nephropathy was established. After the establishment, HE staining, immunohistochemical staining and real-time quantitative PCR were performed to compare the groups. The situation after the successful model creation; observe the two groups of TGF-ß1, P53, microRNA103 and microRNA105 mRNA expression determination and TGF-ß1 and P53 protein expression levels. The success rate of the experimental rat model was 100%; the expression levels of TGF-ß1, P53, and microRNA105 mRNA in the experimental group were significantly higher than those in the control group, and the difference was statistically significant (P<0.05), but the microRNA103 content of the groups was compared. The difference was not statistically significant (P>0.05); after modeling, the expression levels of TGF-ß1 and P53 protein in the experimental group were significantly higher than those in the control group (P<0.05). It was concluded that TGF-ß-mediated P53 and microRNA-related signaling pathways can coordinate with each other and jointly promote the occurrence and development of diabetic nephropathy.

Page(s): 2993-2996

DOI: 10.17582/journal.pjz/20230316100317

Published: Journal: Pakistan Journal of Zoology, Volume: 56, Issue: 6, Year: 2024

Keywords:

p53 , Diabetic Nephropathy , TGFß , microRNArelated signaling pathways

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