Abstract:
As viral resistance to available chemical drugs and virus latency duration leads to worries in herpes simplex virus type 1 and 2 infections treatment, there are growing need for new anti-herpes treatments. Therefore, the present study was aimed to evaluate the antiviral activity of Morus alba extract against herpes simplex virus type 1 and 2 (HSV-1 and 2) in Vero cells model and the antioxidant property of this extract using the DPPH (2,2-diphenyl-1- picryl-hydrazyl-hydrate) free-radical assay. HSV-infected Vero cells and cell-free virus suspensions were treated with M. alba extract, and virus yield and infectivity were quantified by cytopathic effect (CPE) assay. The results indicated that the 50% DPPH radical scavenging property of the extract was 122.4 µg/ml. In addition, the data of the present experiment showed that M. alba extract at 2 µg/ml provided 66.7% inhibition of plaque of HSV-1. In addition, M. alba extract at 4 µg/ml provided 48.6% inhibition of plaque of HSV-2. The extracts showed 100% inhibition against HSV-1 and 2 at 5, 7.5 and 10 µg/ml. These properties suggested that M. alba extract may be used as a topical prophylactic and/or therapeutic agent for herpes infections. However, further studies are required to illuminate the active constituents of this extract which may be useful in developing new anti-herpes drugs.
Page(s):
1563-1569
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Zoology, Volume: 47, Issue: 6, Year: 2015