Abstract:
Fraxinus xanthoxyloides is a small tree of dry hills, commonly known as Afghan ash having leaflets 5-11, ovate lanceolate, toothed with a winged rachis. In this study, both in vitro and in vivo antidiabetic activity of Fraxinus xanthoxyloides bark extract was investigated for the first time by considering it as a potential source of drug candidate. Powder of Fraxinusx anthoxyloidesstem bark was extracted with methanol to obtain the crude extract (FXBM) and the resultant was fractionated with solvents in escalating polarity; n-hexane (FXBH), chloroform (FXBC), ethyl acetate (FXBE) and the residual aqueous fraction (FXBA). The evaporation of respective solvents was done by rotary evaporator, the extract/fractions were dried and stored in refrigerator at 4°C. In vitro investigation of Fraxinus xanthoxyloides bark extract/fractions on inhibition of a-amylase and a-glucosidase activity was assessed through IC50. Among all the extract/fractions, FXBH (IC50 =33.38 µg/ml) showed the powerful inhibition of a-amylase that was comparable to the Acarbose (35.8 µg/ml) as well as FXBH (IC50 =245.6 µg/ml) also showed the powerful inhibition of a- glucosidase comparable to Acarbose (12.53 µg). HPLC-DAD analysis of FXBH showed the presence of Gallic acid, Catechin, Caffeic acid, Ferulic acid and Querctin. In vivo antidiabetic potential of n-hexane fraction of Fraxinus xanthoxyloides bark extract was evaluated through a trial of 15 days on albino rats (Sprague Dawley). Rats were acclimatized and 150 mg/kg of alloxan monohydrate was injected intraperitoneally to induce diabetes after an overnight fasting. After induction of diabetes, different dosage levels of FXBH, 200 mg/kg, 400 mg/kg and Glibenclamide, 5 mg/kg was given orally to rats according to their body weight through feeding tubes. Increase in body weight and decrease in blood glucose level was observed in rats treated with plant extract while blood glucose level was checked with the help of touch glucometer. At the end of trial, rats were sacrificed and blood was collected in tubes and pancreas was stored in formalin. Different biochemical parameters like serum Triglyceride, Lowdensity lipoprotein (LDL) cholesterol (mg/dl), Lipase (mg/dl), Amylase (mg/dl), Alanineaminotransferase (ALT) (mg/dl), Aspartate aminotransferase (AST) (mg/dl), Creatinine (mg/dl),Urea (mg/dl) and C-reactive protein (CRP) (mg/dl) levels were decreased along HBAIC (mg/dl)while serum High-density lipoprotein (HDL) cholesterol (mg/dl) level was increased in ratstreated with Fraxinus xanthoxyloides bark extract. Histopathological analysis of pancreas of rats demonstrated the restoration of beta cells. Hence it was concluded that FXBH possesses a potent antidiabetic potential.
Page(s):
245-245
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Alloxan
,
Histopathology
,
alpha amylase
,
Monohydrate
,
Alpha glucosidase
,
Fraxinus xanthoxyloides