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Evaluation of interactive effect of nanoparticles and salinity (NaCl) stress on Potato (Solanum tuberosum L.) using morphological and biochemical parameters as indicators
Author(s):
1. Anam Rida: Department of Botany, University of Sargodha, Sargodha, Pakistan
2. Aamir Ali: Department of Botany, University of Sargodha, Sargodha, Pakistan
3. Naveed Abbas Nasir: Department of Botany, University of Sargodha, Sargodha, Pakistan
4. Nasim Ahmad Yasin: Horticulture Wing, University of the Punjab, Lahore, Pakistan
5. Zain Ali: Department of Horticulture, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan
6. Muhammad Jamil: Department of Botany, Islamia University Bahawalpur, Pakisatan
7. Naima Huma Naveed: Department of Botany, University of Sargodha, Sargodha, Pakistan
Abstract:
An in-vitro experiment was designed to investigate the interactive effects of NaCl stress and silver nanoparticles on potato (Solanum tuberosum L.). Single nodal sections of two potato cultivars (Metro and Laura) were inoculated in MS media by treating it with various concentrations of NaCl (25, 50, 75, 100, 125 and 150mM). All morphological parameters (shoot length and number, root length and number, leaf number, leaf length and internodal length) were negatively affected under NaCl stress. Biochemical parameters i.e. total soluble proteins, total soluble sugars and activity of nitrate reductase activity were increased in both cultivars. Antioxidant enzymes and amino acids were increased under stress only in Laura. Results indicate that Metro was more tolerant to NaCl stress as compared to Laura. Silver nanoparticles (20 ppm) under salt stress (75mM) increased the number of shoots (1), shoot length (0.74 cm), leaf number (1.4), number of nodes (1.8) and internodal length (0.24 cm) in Metro. In case of Laura the number of shoots (1.2), shoot length (0.94 cm), leaf number (8) and internodal length (0.27 cm) were increased with 20 ppm of silver nanoparticles at 100mM of NaCl concentration. Biochemical parameters i.e. activity of nitrate reductase, antioxidant enzymes and total soluble sugars contents were more increased than salt stress alone in both cultivars. The results indicate that silver nanoparticles overcome the negative impacts caused by NaCl stress on in-vitro cultures of potato and enhanced the overall growth.
Page(s): 167-167
DOI: DOI not available
Published: Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Potato , Invitro , stress , Nanoparticles , Sodium chloride
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