The effect of miR-1468 on Growth Hormone in Pituitary Cells of Yanbian Yellow Cattle | [Pakistan Journal of Zoology • 2021]

Author(s):

1. Tai-hua Jin: College of Agriculture and Forestry Science, Linyi University,Linyi 276005,China

2. An-gang Lou: Agriculture College, Yanbian University,Yanji 133000,China

3. Jiu-xiu Ji: College of Agriculture and Forestry Science, Linyi University,Linyi 276005,China

4. Cheng-dou Cui: Agriculture College, Yanbian University,Yanji 133000,China

5. Long-zheng Yu: Agriculture College, Yanbian University,Yanji 133000,China

6. Li-zeng Guan: College of Agriculture and Forestry Science, Linyi University,Linyi 276005,China

Abstract:

At present, the relationship between miRNAs and GH has been reported. However, the effect of miR1468 on growth hormone (GH) is unknown. The objective of this study was to evaluate effects of miR-1468 on GH in pituitary cells of Yanbian yellow cattle in this study. Real-time quantitative PCR (qPCR), Western blot, Targetscan and RNAhybrid analysis software and Dual-Luciferase reporter gene system was used to analyze the regulation mechanism between miR-1468 and GH. The results showed that the GH mRNA and protein level in pituitary cells of Yanbian yellow cattle could be significantly decreased by adding miR-1468 mimics (P<0.01), while these were significantly increased by adding miR-1468 inhibitor (P<0.05). The results of bioinformatics analysis and double luciferase reporter gene system validation showed that miR-1468 targeted 3'UTR of cAMP responsive element binding protein 1 (CREB1). miR-1468 mimics could significantly inhibit the CREB1 mRNA and protein level (P<0.01), whereas miR-1468 inhibitor could significantly increase the CREB1 mRNA and protein level (P<0.01). This study demonstrated that miR-1468 could regulate GH in pituitary cells by regulating the expression of CREB1 gene. The results of this study will provide a theoretical basis for studying the mechanism of growth and development of animals regulated by miRNAs.

Page(s): 1425-1433

DOI: 10.17582/journal.pjz/20200323080333

Published: Journal: Pakistan Journal of Zoology, Volume: 53, Issue: 4, Year: 2021

Keywords:

Yanbian yellow cattle , Pituitary cells , miR1468 , CREB1

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