De-sizing of Cotton Fabric with Amylase Produced by Indigenously Isolated Bacillus CereusAS2 | [First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book) • 2022]

Author(s):

1. Aneela Rehman: Department of Microbiology, University of Karachi,Karachi,Pakistan

2. Asma Saeed: Department of Microbiology, University of Karachi,Karachi,Pakistan

3. Mujaddadur Rehman: Department of Microbiology, Abbottabad University of Science and Technology,Havelian, Abbottabad,Pakistan

4. Azam Hayat: Department of Microbiology, Abbottabad University of Science and Technology,Havelian, Abbottabad,Pakistan

5. Ibrar Khan: Department of Microbiology, Abbottabad University of Science and Technology,Havelian, Abbottabad,Pakistan

Abstract:

Textile processing is an ever-growing industry that has traditionally used a lot of chemicals, energy and water but due to the environment hazards and high costs of these chemicals, investigation is under way throughout the world to replace chemical de-sizing with economically attractive and eco-friendly bioprocesses. Present study describes the comparison of traditional de-sizing to bio-based de-sizing method and optimization of conditions for fabric de-sizing through crude amylase. In the present study, fermentative de-sizing potential was found maximum at 72 hrs with no surface destruction of fabric. Optimum fabric de-sizing to TEGEWA scale 9 was observed in 5N HCl within 1 hr. Optimization of de-sizing conditions for amylase showed maximum activity at 60°C within 15 hrs, 1000 IU/ml of amylase and 0.5% Triton-X. After process optimization, de-sizing efficacy was checked through weight loss and water absorbance and both parameters were found similar for enzyme and commercially de-sized fabric. Through SEM micrographs enzyme de-sized fabric was found free from any starch particle similar to industrially de-sized fabric which ensure the efficacy of bioprocess.

Page(s): 0-0

DOI: DOI not available

Published: Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022

Keywords:

optimization , Textile , Bacillus , Fermentative desizing , Scanning electron micrograph , Ecofriendly bioprocess

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