Acute Oral Toxicity Assessment of Aqueous Extract of Streblus asper Root in Sprague Dawley Rats according to OECD 420 TG | [Journal of Pioneering Medical Sciences • 2025]

Author(s):

1. Mohd Hafiz Mail: Department of Toxicology, Advanced Medical and Dental Institute, University Sains Malaysia, Bertam, 13200 Pulau Pinang, Malaysia

2. Azman Seeni: Advanced Materials Research Centre, Standard and Industrial Research Institute of Malaysia, Ministry of Trade and Industry, Kulim, 09000 Kedah, Malaysia

3. Farah Anis Shafie: Department of Toxicology, Advanced Medical and Dental Institute, University Sains Malaysia, Bertam, 13200 Pulau Pinang, Malaysia

4. Nozlena Abdul Samad: Department of Toxicology, Advanced Medical and Dental Institute, University Sains Malaysia, Bertam, 13200 Pulau Pinang, Malaysia

Abstract:

The growing interest in the use of medicinal plants for treatment and healthcare necessitates thorough investigations into their safety profiles, many of which remain scarce. This research evaluates the acute toxicity of Streblus asper (SA) root aqueous extract, adhering to the OECD 420 testing guideline. Initially, a preliminary study administered increasing single doses of the extract, ranging from 5 up to 2000 mg/kg body weight, via oral gavage to female Sprague-Dawley rats. Following the determination of an appropriate starting dose, the main study included four additional rats. Over 14 days, subjects were monitored for mortality, toxicity signs, body weight and food consumption, followed by gross pathology analysis at the study's conclusion. The findings revealed no mortality or toxicity signs, consistent weight gain corresponding to food intake and no adverse effects on vital organs. Consequently, the study infers that the LD50 of SA root extract exceeds 2000 mg/kg body weight in rats, indicating a potentially safe profile for therapeutic applications. This research contributes valuable data on the safety of medicinal plants, supporting their integration into healthcare practices.

Page(s): 48-52

DOI: 10.47310/jpms2025140808

Published: Journal: Journal of Pioneering Medical Sciences, Volume: 14, Issue: 8, Year: 2025

Keywords:

LD50 , Acute oral Toxicity , OECD 420 TG , Streblus asper

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