Utilization of Fermentatively Produced Antimicrobial Peptide-Silver Nanoconjugates against Selected Bacterial Pathogens | [International Journal of Agriculture and Biology • 2024]

Author(s):

1. Muhammad Usman Ahmad: Dr. Ikram-ul-Haq Institute of Industrial Biotechnology, Government College University,Lahore-54000,Pakistan

2. Kaynat William: Department of Microbiology, Government College University,Lahore-54000,Pakistan

3. Sikander Ali: Department of Microbiology, Government College University,Lahore-54000,Pakistan

4. Fareeha Akhtar: University Diagnostic Laboratory, Institute of Microbiology, University of Veterinary and Animal Sciences,Lahore-54000,Pakistan

5. Saba Sana: Department of Microbiology, Government College University,Lahore-54000,Pakistan

6. Sundas Sharif: Dr. Ikram-ul-Haq Institute of Industrial Biotechnology, Government College University,Lahore-54000,Pakistan

Abstract:

In an attempt to curb the health and environmental hazards of chemical food preservatives, a continuous search for natural alternatives is going on continuously. Despite many reports on antagonistic activity of antimicrobial peptides (AMPs) or bacteriocins, certain shortcomings including narrow spectrum of activity and requirement of high dosage hinder it from becoming a practicable approach. Bioconjugation of AMPs with silver nanoparticles is proposed as a resourceful means for enhancing the antimicrobial potential of AMPs. The present study was conducted for utilization of biogenically synthesized antimicrobial peptide (AMP)-silver nanoconjugates produced from Lactic Acid Bacteria (LAB). For this purpose, a culture of Lactobacillus sp. was isolated, partially purified and its activity was assessed. Biogenic synthesis of AMP-nanoconjugates was carried out along with optimization of concentration ratio, temperature and pH. Lastly, antimicrobial activity of AMP-AgNPs was evaluated using disc diffusion assay against six indicator bacteria including Escherichia coli, Salmonella sp., Staphylococcus aureus, multi drug resistant (MDR) Klebsiella sp., Bacillus subtilis and Methicillin-resistant Staphylococcus aureus. Results of in vitro activity showed synergistic antibacterial efficacy of AMP-AgNPs as their activity was considerably greater than that of individual activity of partially purified AMP. The biogenically synthesized AMP-nanoconjugates showed remarkable antibacterial potential against all bacteria especially against MRSA and MDR Klebsiella sp., which is a steppingstone in use of these composites against drug resistant microorganisms in wide ranging industrial applications.

Page(s): 137-146

DOI: 10.17957/IJAB/15.2125

Published: Journal: International Journal of Agriculture and Biology, Volume: 31, Issue: 2, Year: 2024

Keywords:

lactic acid bacteria , Green synthesis , Antimicrobial peptides , Bacteriocinnanoconjugates AgNPs , bacteriocins

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views