Acquisition of Desiccation Tolerance in Faba Bean (Vicia Faba) as Affected by Maturity Stages, Harvested Organ Type, and Drying Method. | [Pakistan Journal of Botany • 2019]

Author(s):

1. ALI MORADI: Department of Agronomy and Plant Breeding, Yasouj University, Yasouj, Iran

2. Ezatollah Karimi Arpanahi: Department of Agronomy and Plant Breeding, Yasouj University, Yasouj, Iran

Abstract:

A field experiment was carried out to investigate the effects of harvesting organ type and drying method on the acquisition of desiccation tolerance during different development stages of faba bean (cv. Shami) seed. The plants were harvested in three pod developmental stages including Mid-Full Sized Seeds (MS), Full-Sized Seed (FS), and GreenishYellow pod (GY) were divided into three organ forms (depodded, podded detached, and podded attached to the plants). Finally, they were dried using two methods including shade-drying and sun-drying. An extra harvesting was also done at yellowish-brown (YB) stage according to the conventional harvest of local farmers as a control. The results showed that as the seeds become more mature, from MS stage to GY stage, germination percentage (GP), natural seedling percentage (NS), content of soluble sugars (SS), and activity of catalase (CAT) and glutathione (GR) enzymes was increased while the mean germination time (MGT) and ascorbate peroxidase (APX) was decreased. From GY stage to YB stage, MGT and soluble protein (SP) were increased while other were traits decreased. In MS, FS, and GY stages, the highest and lowest GP, NS, SS, CAT, and GR enzymes activity were observed in the podded attached shade-dried seed and depodded sun-dried seed treatments, respectively. We found that among all treatments, MGT had an opposite behavior. Overall, the seeds showed desiccation tolerant behavior, a higher amount of SS, CAT, and GR, and lower APX activities compared to those of sensitive seeds. Therefore, it was concluded that harvesting, organ type, and drying method play important roles in the acquisition of desiccation tolerance and promoting seed quality, especially in the early stages of maturity of faba bean seeds.

Page(s): 37-47

DOI: 10.30848/PJB2019-1(1)

Published: Journal: Pakistan Journal of Botany, Volume: 51, Issue: 1, Year: 2019

Keywords:

faba bean , seed germination , Antioxidant enzyme , Drying method , Seed maturity

References:

[1] Aizadeh-Benab , G.,A.Tobeh,K.Ghassemi-Golezani,S. SadegzadehHemayati,A, 2006.Investigation of different sowing and harvesting dates effect on yield and quality of monogerm sugar beet seed,Paj. Sazandagi (in Persian with English abstract) 2 33 -42

[2] Angelovici , R.,G.Galili,A.R.Fernie,A.Fait, 2010.Seed desiccation: a bridge between maturation and germination, 15 211 -218

[3] Anonymous, 2010.Published by the international seed testing Association, -

[4] Arrigoni , O.,L.D.Gara,F.Tommasi,R.Liso, 1992.Changes in the ascorbate system during seed development of Vicia faba L. Plant Physiol, 99 235 -238

[5] Bailly , C.,C.Audigier,F.Ladonne,M.H.Wangner,F.Coste,F.Corbineau,D.Come, 2001.Changes in oligosaccharide content and antioxidant enzyme activities in developing bean seeds as related to acquisition of drying tolerance and seed quality,Exp 52 701 -708

[6] Bailly , C.,J. Leymarie , A.,Lehner , S.,Rousseau , D.,Côme and F.,Corbineau, 2004.Catalase activity and expression in developing sunflower seeds as related to drying, 55 475 -483

[7] Black , M.,F.Corbineau,H.Gee,D.Come, 1999.Water content, raffinose and dehydrins in the induction of desiccation tolerance in immature wheat embryos,Plant 120 463 -471

[8] Black , M.,F.Corbineau,M.Grzesik,P.Guy,D.Come, 1996.Carbohydrate metabolism in the developing and maturing wheat embryo in relation to its desiccation tolerance,Exp 47 161 -169

[9] Blackman , S.A.,R.L.Obendorf,A.C.Leopold, 1992.Maturation proteins and sugars in desiccation tolerance of developing soybean seeds,Plant 100 225 -230

[10] Bradford , M., 1976.A rapid and sensitive method for the quantitation of protein utilizing the principle of protein dye binding,Analyt 72 248 -25

[11] Brenac , P.,M. Horbowicz , S.M.,Downer , A.M.,Dickerman , M.E.,Smith and R.L.,Obendorf, 1997.Raffinose accumulation related to desiccation tolerance during maize (Zea mays L.) seed development and maturation,Plant Physiol 150 481 -488

[12] Cakmak , I.,W.Horset, 1991.Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean,Plant 83 463 -468

[13] Corbineau , F.,M.A.Picard,J.A.Fougereux,F.Ladonne,D.Come, 2000.Effects of dehydration conditions on desiccation tolerance of developing pea seeds as related to oligosaccharide content and cell membrane properties,Seed Sci. Res 10 329 -339

[14] Dayal , A.,N. R.Rangare,A.Kumar,M.Kumari, 2015.Effect of physiological maturity on seed quality of maize (Zea mays,Forage. Res. 40 1 -6

[15] Dean , J.A., 1985.Legends handbook of chemistry, 5 96 -101

[16] Doijode , S.D., 1990.Germinability and longevity of onion seeds under different drying methods,Progress. Hort. 22 117 -120

[17] Ellis , R.A.,E.H.Roberts, 1981.The quantification of ageing and survival in orthodox seeds,Seed Sci.Technol 9 373 -409

[18] Ellis , R.H.,R.D.Hong,E.H.Roberts, 1987.The development of desiccation tolerance and maximum seed quality during maturation in six grain legumes,Annals Bot. 59 23 -29

[19] Ennen , R.D., 2011.Earlier harvest and drying of soybean seed within intact pods maintains seed quality,Graduate Thesis -

[20] Eskandari , H., 2012.Seed quality changes in cowpea (Vigna sinensis) during seed development and maturation,Seed Sci. Technol 40 108 -112

[21] Fu , J.R.,X.Q.Yang,X.C.Jiang,J.X.He,S.Q.Song, 1997.Heat stable proteins and desiccation tolerance in recalcitrant and orthodox seeds,Basic and Applied Aspects of Seed Biology 30 705 -713

[22] Gara , L.D.,M.C. de Pinto , V.M.C.,Moliterni and M.G.D. Egidio, 2003.Redox regulation and storage processes during maturation in kernels of Triticum durum, 54 249 -258

[23] Garnczarska , M.,W.Bednarski,M.Jancelewicz, 2009.Ability of lupine seeds to germinate and to tolerate desiccation as related to changes in free radical level and antioxidants in freshly harvested seeds,Plant Physiol. Bioch. 47 56 -62

[24] Ghasemi-Golezani , K.,B.Roozbeh, .Changes in seed quality of chickpea cultivars under salinity stress,Res. on Crops 12 778 -782

[25] Ghasemi-Golezani , K.,R.Mazloomi-Oskooyi, 2008.Effect of water supply on seed quality development in common bean (Phaseolus vulgaris),Int. Plant Prod 2 117 -124

[26] Ghasemi-Golezani , K.,Z.Tajbakhsh,Y.Raey, .Seed development and quality in maize cultivars,Not. Bot. Hort. Agro. Cluj-Napoca 39 178 -182

[27] Gorecki , R.J.,A.I.Piotrowicz-Cieslak,L.B.Lahuta,R.LObendorf, 1997.Soluble carbohydrates in desiccation tolerance of yellow lupine seeds during maturation and germination,Seed Sci. Res 7 107 -115

[28] Gurusamy , C.,C.P.Thiagarajan, 1998.The pattern of seed development and maturation in cauliflower (Brassica oleracea L. var,Phyton; Annales Rei Botanicae 38 295 -268

[29] Harington , J.F.,T.T. SeedBiology, 1972.Seed storage and longevity, 145 -245

[30] Hay , F.,R.Probert, 1995.Seed maturity and the effects of different drying conditions on desiccation tolerance and seed longevity in fox glove (Digitalis purpurea L.), 76 639 -647

[31] Hendry , G.A.F., 1993.Oxygen free radical processes and seed longevity,Seed Sci. Res 3 141 -153

[32] Hincha , D.K.,E.Zuther,A.G.Heyer, 2003.- Biomem,Bioch. Bioph. Acta (BBA 1612 172 -177

[33] Huang , H. and S.,Sung, 2013.Change in desiccation tolerance of maize embryos during development and germination at different water potential PEG-6000 in relation to oxidative process,Plant Physiol. Bioch. 68 61 -70

[34] Irigoyen , J.J.,D.W.Emerich,M.Sanchesdiaze, 1992.Water stress induces changes in concentration of proline and total soluble sugars in nodulated alfalfa plant,Physiol. Plant 84 55 -60

[35] Kermode , A.R.,J.,G. Galili., 1995.Seed development and germination, 273 -332

[36] KingstonSmith,A.H.,J.Harbinson,C.H.Foyer, 1999.Acclimation of photosynthesis, H2O2 content and antioxidants in maize (Zea mays) grown at sub‐optimal temperatures,Plant Cell. Environ. 22 1071 -1083

[37] Koster , K.L.,A.C.Leopold, 1988.Sugars and desiccation tolerance in seeds,Plant 88 829 -832

[38] Lehner , A.,C.Bailly,B.Flechel,P.Poels,D.Come,F.Corbineau, 2006.Changes in wheat seed germination ability, soluble carbohydrate and antioxidant enzyme activities in the embryo during the desiccation phase of maturation,Cereal Sci. 43 175 -182

[39] Lehner , A.,N.Mamadou,P.Poels,D.Come,C.Bailly,F.Corbineau, 2008.Changes in soluble carbohydrates, lipid peroxidation and antioxidant enzyme activities in the embryo during ageing in wheat grains,Cereal. Sci. 47 555 -565

[40] Leopold , A.C.,R.G.,J.RCumming, 1990.Stress responses in plants adaptation and acclimation mechanisms,NewYork 57 -86

[41] Leprince , O.,N.M.Atherton,R.Deltour,G.Hendry, 1994.The involvement of respiration in free radical processes during loss of desiccation tolerance in germinating Zea mays (an electron paramagnetic resonance study),Plant Physiol 104 1333 -1339

[42] Miles , D.F.,D.M. TeKrony and D.B. Egli, 1988.Changes in viability, germination, and respiration of freshly harvested soybean seed during development,Crop Sci. 28 700 -704

[43] Moller , I.M., 2001.Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species,Ann. Rev. Plant Biol 52 561 -591

[44] Mullineaux , P.M.,G.P.Creissen,J.G., 1997.Oxidative stress and the molecular biology of antioxidant defenses, 667 -713

[45] Nakano , Y.,K.Asada, 1981.Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts,Plant. Cell Physiol 22 867 -880

[46] Nedeva , D.,A.Nikolova, 1997.Desiccation tolerance in developing seeds,Plant 23 100 -113

[47] Obendorf , R.L., 1997.Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance,Seed Sci. Res 7 63 -74

[48] Rowland , G.G.,D.B.Fowler, 1997.Factors affecting selection for seed coat thickness in Fababeans (Vicia faba L),Crop. Sci. 17 88 -90

[49] Samarah , N.H., 2005.Effect of drying methods on germination and dormancy of common vetch (Vicia sativa L.) seed harvested at different maturity stages,Seed Sci. Technol 33 733 -740

[50] Samarah , N.H., 2006.Effect of air-drying immature seeds in harvested pods on seed quality of common vetch (Vicia sativa L.),New Zealand Agri. Res. 49 331 -339

[51] Samarah , N.H.,M.M.Al-Mahasneh,H.Z.Ghosheh,A.M.Alqudah,M.Turk, 2010.The influence of drying methods on the acquisition of seed desiccation tolerance and the maintenance of vigour in wheat (Triticum durum),Seed Sci. Technol 38 193 -208

[52] Samarah , N.H.,R.E.Mullen,S.Goggi,A.Gaul, 2009.Effect of drying treatment and temperature on soybean seed quality during maturation,Seed Sci. Technol 37 469 -473

[53] Sanhewe , A. J,R. H. Ellis, 1996.Seed development and maturation in Phaseolus vulgaris: Ability to germinate and to tolerate desiccation,Journal Exp. Bot. 47 949 -958

[54] Scandalios , J.G., 1997.Oxidative stress and the molecular biology of antioxidant defenses, -

[55] Smirnoff , N., 1993.The role of active oxygen in the response of plants to water deficit and Desiccation, 125 27 -58

[56] Smith , M.T.,P.Berjak,J.,G. Galili. SeedDevelopment, 1995.Deteriorative changes associated with the loss of viability of stored desiccationtolerant and -sensitive seeds, 701 -704

[57] Tekrony , D.M.,D.Egli,D.B.Balles,J.Stuky,L.Tomas, 1984.Effect of date of harvest maturity on soybean seed quality and Phomopsis sp. seed infection,Crop. Sci. 14 189 -193

[58] Verdier , J.,D.Lalanne,S.Pelletier,I.Torres-Jerez,K.Righetti,K.Bandyopadhyay,O.Leprince,E.Chatelain,B.L. and Vu,J.Gouzy, 2013.A regulatory network-based approach dissects late maturation processes related to the acquisition of desiccation tolerance and longevity of Medicago truncatula seeds,Plant 163 757 -774

[59] Vertucci , C.W.,J.M.Farrant,J.,G. Galili., 1995.Seed development and germination, 237 -271

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