Abstract:
Objective: The effectiveness of chewing tobacco in left coronary artery of mice. Place and Duration: From July 2015 to June 2016 at the Department of Anatomy LUMHS, Jamshoro Methodology: Total 20 Healthy male mice were obtained from the Animal House Sindh Agricultural University Tandojam. These mice were categorized into two groups each of 10 animals. The groups were labeled as Control Group A and Experimental Group B. Experimental Group-B was provided chewing tobacco 5mg mixed with standard diet along with clean water ad libitum, whereas Group A, The Control was provided standard diet and clean water ad libitum. After 30 days, the animals were sacrificed by cervical dislocation and their left coronary vessels were dissected out for histological analysis. Results: Left coronary artery weight in control and experiment groups was measured as 0.35 ± 0.01g/cm3 and 0.39± 0.02 g/cm3, which was observed as significant (P value < 0.01). However, the thickness of coronary artery was also increased in chewing tobacco users. Histological examination of the coronary artery control mice showed normal tunica intima, media and adventitia are visible without any narrowing of lumen. Histological abnormalities were noted in the tobacco chewing mice. Various lesions primarily of atheroma type, ulceration, lumen narrowing, early plaque, foamy macrophages, leukocytes, thick internal matrix, fatty lesions and a complicated atheromatous plaque with areas of necrosis were noted in the mice model of experimental animals. Conclusion: The present study reported the deleterious effects of chewing tobacco in left coronary arteries of experimental mice. Lesions such as atheroma, ulceration, lumen narrowing, early plaque, foamy macrophages, leukocytes, thick internal matrix, fatty lesions and a complicated atheromatous plaque with areas of necrosis were noted in the mice model.
Page(s):
177-180
DOI:
DOI not available
Published:
Journal: Annals of the Pakistan Institute of Medical Sciences, Volume: 13, Issue: 2, Year: 2017