Abstract:
Obstruction of blood vessels with clots causes acute myocardial infarction and ischemic stroke which are the leading causes of death. Thrombolytic agents are administered to liquefy the blood lump. Production of fibrinolytic enzyme from Oidiodendron flavum, was carried out in this research work. Indigenous soil and compost samples were collected. After processing, samples were inoculated on PDA (peptone dextrose-agar media) plates and Fibrin salt basal media was used for isolation of Oidiodendron flavum. Cottonseed cake was used as the substrate for the isolated strain. The isolated strain (IBBM-1) of Oidiodendron flavum were used for the production and isolation of fibrinolytic enzyme. To seek the optimum activity, different kinetic and thermodynamic parameters like pH, temperature and molecular weight were applied on the purified enzyme. The enzyme produced by the isolated strain resulted in 300 U/ml with 53.57 U/mg specific activity, 5.6 mg/ml protein, 3.78-fold purification and 82.6% recovery. Fibrinolytic enzyme produced from the isolated strain exhibited activity within the pH range of 7.0 to 8.0 while it gave its best performance at pH 8.0. Thermal stability at 37?C was shown by the fibrinolytic enzyme. For this purpose, growth of Oidiodendron flavum was optimized at various parameters (pH, temperature, substrate concentration and fermentation period). By using these optimized conditions maximum growth of Oidiodendron flavum would be obtained and good quality fibrinolytic enzyme with cheap cost can be produced at industrial level.
Page(s):
95-95
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023