A study of the interaction between H. Pylori mice passage strains and gastric epithelial cells. | [Pakistan Journal of Pharmaceutical Sciences • 2018]

Author(s):

1. Inayat U Rahman: GandharaCollege of Pharmacy, Gandhara University,Peshawar,Pakistan

2. Muhammad Idrees: Department of Pathology, Khyber Medical College (KMC),Peshawar,Pakistan

3. Mohammad Waqas: Department of Pathology, Khyber Medical College (KMC),Peshawar,Pakistan

4. Abdul Karim: Department of Genetics, Microbiology and Toxicology, Stockholm University, Sweden

Abstract:

Helicobacter pylori (H. pylori) infections are very serious health problem that are further worsened by increasing/developing resistance to the current antibiotics. Therefore, new therapeutic agents are needed for H. pylori eradication. Use of a CD46 derived peptide (P3) as bactericidal agent against H. pylori has shown high activity rate in vivo and this study examines the changes in H. pylori features in response to effect of P3 treatment.AGS cells were infected with H. pylori wild type strain 67:21 and its mice passage strains (P3 treated and untreated strains) and further examined using immunoblotting assay, FACS and Urease activity analysis. Comparatively we found increased level of Urease alpha subunit A (UreA) and alkyl hydroperoxide reductase C (AhpC) proteins for P3 treated strain of H. pylori than its wild type or untreated strain after infection of AGS cells. Conclusion These results suggest that there might be a high rate of adherence to host cells for the P3 treated passage strain than untreated or wild type strain. Our findings also indicate that either adhesins are being changed or H. pylori interaction to the host cells is affected after P3 treatment.

Page(s): 769-775

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 31, Issue: 3, Year: 2018

Keywords:

bactericidal , CD46 derived peptide P3 , pylori

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