Detection of small-sized DNA fragments in a glassy nanopore by utilization of CRISPR- Cas12a as a converter system | [Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23 • 2023]

Author(s):

1. Muhammad Asad Ziaee: School of Mechanical and Electrical Engineering, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, China; Hamdard University, Faculty of Engineering Sciences & Technology- FEST

2. Shumin Zhang: School of Mechanical and Electrical Engineering, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, 510006, China

3. Jiahai Wang: School of Mechanical and Electrical Engineering, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, 510006, China

Abstract:

The fabrication of nanopores with a matched pore size, and the existence of multiple interferents makethe reproducible detection of small-sized molecules by means of solid-state nanopores still challenging. A useful method to solve these problems is based on the detection of large DNA nanostructures relatedto the existence of small-sized targets. In particular, a DNA tetrahedron with a well-defined 3D nanostructureis the ideal candidate for use as a signal transducer. Here, we demonstrate the detection of anL1-encoding gene of HPV18 as a test DNA target sequence in a reaction buffer solution, where longsingle-stranded DNA linking DNA tetrahedra onto the surface of the magnetic beads is cleaved by a targetDNA-activated CRISPR-cas12 system. The DNA tetrahedra are subsequently released and can be detectedby the current pulse in a glassy nanopore. This approach has several advantages: (1) one signal transducercan be used to detect different targets; (2) a glassy nanopore with a pore size much larger than the targetDNA fragment can boost the tolerance of the contaminants and interferents which often degrade theperformance of a nanopore sensor.

Page(s): 353-353

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023

Keywords:

glassy nanopore , CRISPRCas12a

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