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Transfer of cucumber mosaic virus resistance into hybrids of tomato.
Author(s):
1. Muhammad Yussouf Saleem: Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan
2. Khalid Pervaiz Akhtar: Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan
3. Qumer Iqbal: Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan
4. Muhammad Asghar: Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan
5. Muhammad Shoaib: Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan
Abstract:
A tomato male sterile line TMS1, tolerant to cucumber mosaic virus (CMV) was crossed with 5 high yielding but CMV susceptible lines namely Naqeeb, Money Maker, B23, B25 and Peto-86 to transfer the resistance into F1 hybrids. The entire breeding material was subjected to mechanical inoculation followed by double antibody sandwich procedure (DAS-ELISA) to confirm the presence or absence of CMV. Data was recorded on the percentage of disease transmission, mean latent period, average disease severity and yield and yield related traits in field. TMS1 was reconfirmed ‘tolerant’ through mechanical inoculation but ‘resistant’ in natural filed conditions. Hybrids did not express tolerance level at par with TMS1 in mechanical evaluation. However, they showed at par response under field evaluation except hybrid TMS1 × B25. Hybrids TMS1 × Naqeeb, TMS1 × MM, TMS1 × B23, TMS1 × B25 and TMS1 × Peto-86 showed better tolerance to CMV over standard hybrids T-1359 and Salar. Line TMS1 proved to be a valuable donor of CMV resistance in cultivated tomato.
Page(s): 671-675
DOI: DOI not available
Published: Journal: Pakistan Journal of Agricultural Sciences, Volume: 52, Issue: 3, Year: 2015
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