Abstract:
A pot experiment was conducted to check the effect of foliarly applied ascorbic acid on two barley (Hordeum vulgare L.) genotypes under lead chloride (PbCl2) stress. An experiment was conducted at Old Botanical Garden, the University of Agriculture Faisalabad, Pakistan. Barley seeds were taken from Ayub Agriculture Research Center Faisalabad and were sowed into the plastic pots filled with thoroughly washed sand. Two levels of lead chloride (0µM and 500µM) were applied with full strength Hoagland solution after four week of germination. Then two ascorbic acid levels (0ppm and 2ppm) were applied by foliar spray on very next day of salt stress. The experiment was carried out by using completely randomized design (CRD) with three replications. Experimental parameters such as physiological, biochemical, morphological, enzymatic and non-enzymatic antioxidants, MDA and H2O2 level were determined. Results showed that growth parameters (like shoot fresh and dry weight, root fresh and dry weight, shoot length and root length, leaf length and leaf area), physiological parameters (like chl. a, chl. b, total chl. carotenoids and anthocyanin) and H2O2 level were reduced under lead stress. Lead stress also decreased the CAT activity while increased the SOD and POD activity, total soluble proteins, total soluble sugars, free proline, and level of MDA. However, foliarly applied ascorbic acid ameliorated the negative effects of lead stress on barley varieties. Data were analyzed under three way ANOVA techniques by using COSTAT software.
Page(s):
17-17
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
barley
,
germination
,
Ascorbic Acid
,
and physiological parameters