Author(s):
1. Ayesha Gulzar:
Department of Biochemistry, IBBB, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
2. Fatima Ismail:
Department of Biochemistry, IBBB, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Abstract:
Acremonium sclerotigenum (A. sclerotigenum) is a rare opportunistic pathogen that has not been reported previously from Southern Punjab Pakistan. Goal of this study was to determine the morphological and biochemical characterization of A. sclerotigenum. Fungi were isolated during the clinical isolation of dermatophitic fungal species. A. sclerotigenum was analyzed for molecular identification, phenotypic diversity by microscopic examination, humidity, oxidative, osmotic stress adaptability by culture characteristics and for protease qualitative and quantitative assay. This study also determined the production and activity level of lipase in A. sclerotigenum. Antifungal drug susceptibility of A. sclerotigenum against terbinafine was determined. A. sclerotigenum was genetically identified by sequencing of ITS region. The morphological examination revealed hyaline, septate hyphe giving rise to conidiophores with numerous micro conidias. A. sclerotigenum produced off white, smooth cottony colonies on sabouraud dextrose agar media and white, smooth creamy colonies on oat meal agar media. A. sclerotigenum showed optimum growth at high humidity. Study revealed that A. sclerotigenum does not have oxidative and osmotic stress tolerance as supported by phenotypic results. Biochemical characterization was made on the basis of enzyme production and their activity. Study revealed that A. sclerotigenum does not have significant protease activity. Moderate level of lipase is secreted by A. sclerotigenum. It has very low MICs for terbinafine. A. sclerotigenum found sensitive against terbinafine as supported by phenotypic results. Study suggested that A. sclerotigenum has adaptability to physiological factors such as humidity and temperature, also the lipase secretion contribute to virulence of the fungi.
Page(s):
88-88
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
oxidative stress
,
Microscopic examination
,
relative humidity
,
Biochemical characterization
,
Pathogenic fungi
,
Acremonium sclerotigenum
References:
References are not available for this document.
Citations
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