Activation of the PI3K/Akt pathway is essential for the survival of Brucella melitensis 16M In vitro and in vivo. | [Pakistan Veterinary Journal • 2015]

Author(s):

1. Junbo Zhang: College of Animal Science and Technology, Shihezi University, Shihezi 832003, China; College of Biology, Agriculture and Forestry, Tongren University, Tongren, 554300, Guizhou, China

2. Shuanghong Yin: College of Animal Science and Technology, Shihezi University, Shihezi 832003, China; College of Material and Chemical Engineering, Tongren University, Tongren, 554300, Guizhou, China

3. Fei Guo: College of Medicine, Shihezi University, Shihezi, 832003, China

4. Zhiqing Li: College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

5. Chuangfu Chen: College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

6. Hui Zhang: College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

Abstract:

Brucellosis is a zoonotic pathogen that causes both animal and human diseases. Brucella pathogenesis depends on the bacterial ability of inhibiting apoptosis and establishes a replicative niche inside host cells. The PI3K/Akt pathway regulates various cellular activities (cell growth, survival, and apoptosis). However, the role of the PI3K/Akt pathway in the survival of 16M in RAW264.7 macrophages remains largely unknown. In our report, we demonstrated that the PI3K/Akt pathway was activated by 16M in RAW264.7 macrophages. We found that 16M infection induced the phosphorylation of both Thr-308 and Ser-473 of Akt in a time-dependent manner. This phosphorylation was inhibited by LY in a dosedependent manner. Inhibition of PI3K/Akt with LY significantly reduced the internalisation and replication of 16M and induced 16M-dependent inhibition of apoptosis, and induced Th1 and proinflammatory responses both in vitro and in vivo and protected mice against 16M infection. These results indicated that the PI3K/Akt pathway plays an important role in 16M survival both in vitro and in vivo, which will help to unravel the pathogenic mechanisms of 16M.

Page(s): 7-12

DOI: DOI not available

Published: Journal: Pakistan Veterinary Journal, Volume: 35, Issue: 1, Year: 2015

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