Abstract:
Opuntia dillenii(Nagphana) is one of the edible plants, its cladodes and fruit are rich sources of vitamins, minerals, amino acid and betalins. It is traditionally used against various inflammatory diseases worldwide including Pakistan. Therefore, the present study aimed to provide scientific evidence to its traditional uses. O. dillenii cladode derived methanol extract, its fractions, and pure compounds: opuntiol and opuntioside. were evaluated for toxicological assessment including subacute toxicity assay followed by determination of their hematological, biochemical parameters and histological examination of liver and kidney. Cytotoxicity was evaluated by using rat neutrophils viability (5 x 106 cells/ mL) and HEK-293 human cells using MTT assay. In genotoxicity assay rat bone marrow micronucleus and cytochalasin-B block micronucleus (CBMN) test were performed via DNA isolation. For anti-inflammatory effect: ear edema and carrageenan-induced peritonitis was performed followed by determination of inflammatory markers such as Prostaglandins (PGE2), leukotrienes (LTB4) and cytokines[tumor necrosis factor (TNF-a), interleukins IL-1 and -6]. In analgesic assays the acetic acid, formalin and hot plate-induced pain responses in mice were also observed. O. dillenii test agents did not elicit any visible behavioral changes or mortality,also displayed non-significant difference (p< 0.05) in hematological, biochemical and histological studies. Moreover, test agents were found to be non-cytotoxic. Consistently, in genotoxic assay test agents were also depicted non- significant in micronucleus induction. Test agents also showed antiinflammatory effect, among which opuntiosidewas most effective in reducing PGE2 levels and opuntiol most effectively suppressed the TNF-a, IL-1ß and IL-6 levels. In analgesic assays the acetic acid, formalin and hot plate-induced pain responses in mice were also reduced by O. dillenii derived test agents with opuntioside being most effective. Results obtained in this study suggest that edible O. dillenii derived test agents are relatively safe and exhibited anti-inflammatory and analgesic activity via multiple mechanism of action and provide justification to its traditional use against inflammation and may serve as better candidate in drug discovery program.
Page(s):
187-187
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023