Objective: To examine the level and effect of work-related stress on psychological distress among private university teachers of Pakistan during COVID-19. Methodology: The study was web-based and cross-sectional in which 300 Google forms were disseminated online using The Work-Stress Question naire ( 2 1 - items ) and Kessler Psychological Distress Scale (10 items), respectively. A total of 272 university teachers were included. Results: The level of work stress and psychological distress was found to be high, especially during the period of online lectures Moreover, a positive significant relationship was found between both variables indicated by 2.263 tvalues significant at 0.05 level of significance and variance of 62% in psychological distress. Conclusion: The change sin the work environment and conditions that occurred due to COVID-19 had a strong significant adverse effect on university teachers . Formulation of psychological interventions is required to mitigate the psychological effects of work-related stress in them.