Background: Central corneal thickness and corneal endothelial morphology was studied along it's relationship with age and sex. Objective: To determine the normative data of central corneal thickness and endothelial morphology in our population. Methodology: This is a Cross sectional study. The study was done at Ophthalmology Department, FRPMC in accordance with the declaration of Heliniski from March 2021 to August 2021 with approval from Institutional review board. All healthy individuals aged 20 to 70 years presenting with any ocular complain but no corneal pathology were included. Individuals with any systemic and ocular co morbidity were excluded. After complete ocular examination corneal specular microscopy was done using REXAM SPM-700 corneal specular microscope. In our study we analysed CCT, ECD, MCA, CV and hexagonality. The data was collected and recorded on Excel spreadsheet and then transformed to SPSS version 22 for statistical analysis. Results: 724 eyes from 362 individuals were studied for CCT and endothelial morphology using Rexam SPM-700 microscope. There were 241(66.6%) males and 121(33.4%) females with mean age 38.33±10.17 years. Among 724 eyes we found CCT (508.43±33.4um), ECD (2565.12±254.15cell/mm2), AVG (393.81±40.72um2), CV (39.54±6.43%) and Hexa (46.18±6.64%). Statistically there was no significant difference among right and left eyes of study population as well as gender wise. There is statistically significant inverse correlation of ECD with age (p<0.00, r:-0.19). However, statistically insignificant inverse correlation is found for CV and Hexa with age (p<0.17, r:-0.05) (p<0.70, r:-0.014) respectively. Conclusion: Our study reports the normative corneal endothelial morphology and thickness in Pakistani population attending FRPMC Ophthalmology Department. We also found that Central corneal thickness and Endothelial cell density decreases with aging and no statistical difference gender wise.