Zinc finger X-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells. | Pakistan Journal of Medical Sciences 2013

Author(s)

Rui Jiang Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun, PR China

Zhong-Li Gao Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun, PR China

Mei Sun The Second Hospital of Jilin University, Ziqiang Street, Changchun, PR China

Xing-Yi Zhang Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun, PR China

Jin-Chengwang Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun, PR China

Han Wu Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun, PR China

Abstract

Objective: To investigate the role of Zinc finger X-chromosomal protein (ZFX) in oncogenesis of Osteosarcoma tumor. Methods: Here, we first conducted an expression analysis of ZFX in Osteosarcoma cell lines. Then, we constructed ZFX-specific small interfering RNA (siRNA)-lentiviral vector that is capable of effectively inhibiting the expression of ZFX gene in human Osteosarcoma Saos-2 cells, and investigated systemically the impacts of ZFX silence on the growth and invasive ability of the cancer cells in vitro. Furthermore, we determined the effects of ZFX knockdown on the cell cycle distribution and apoptosis of Saos-2 cells. Results: We found that ZFX inhibition resulted in significantly impaired proliferation and colony formation as well as mitigated invasiveness of Saos-2 cells. Importantly, si-ZFX infected cells exhibited a greater portion of cells at G1 phase, but a minor portion of S and G2/M phase cells. Moreover, a greater portion of sub-G1 apoptotic cells was observed in si-ZFX infected cells. Conclusions: These results strongly suggest that ZFX is a novel proliferation regulator that promotes growth of Osteosarcoma cells, and downregulation of ZFX expression induces growth suppression of Saos-2 cells via arrested G0/G1 phase cell cycle and apoptosis pathways, thereby indicating that ZFX may serve as a new molecular target for Osteosarcoma tumor therapy.

Publication Details

Page(s) 997-1002

DOI DOI not available

Published Journal: Pakistan Journal of Medical Sciences, Volume: 29, Issue: 4, Year: 2013

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