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A Ca+2 INDEPENDENT PULLULANASE FROM Bacillus licheniformis AND ITS APPLICATION IN THE SYNTHESIS OF RESISTANT STARCH
Author(s)
Anum Khan Department of Microbiology, Quaid-i-Azam University,Islamabad-45320,Pakistan
Muhammad Irfan Department of Microbiology, Quaid-i-Azam University,Islamabad-45320,Pakistan
Ubaid Rahman Department of Microbiology, Quaid-i-Azam University,Islamabad-45320,Pakistan
Farah Azhar Department of Microbiology, Hazara University Mansehra,Pakistan
Aamer Ali Shah Department of Microbiology, Quaid-i-Azam University,Islamabad-45320,Pakistan
Malik Badshah Department of Microbiology, Quaid-i-Azam University,Islamabad-45320,Pakistan
Fariha Hasan Department of Microbiology, Quaid-i-Azam University,Islamabad-45320,Pakistan
Fazal ur Rehman Department of Microbiology, University of Balochistan,Quetta,Pakistan
Zulfiqar Ali Malik Institute of Microbiology, Shah Abdul Latif University,Khairpur,Pakistan
Samiullah Khan Department of Microbiology, Quaid-i-Azam University,Islamabad-45320,Pakistan
Abstract
Polysaccharides including resistant starch can be categorized as a part of dietary fiber and used as an important prebiotic. Like soluble fibers, resistant starch also has a number of physiological effects which have been proved to be beneficial for health. Enzymatically modified starch with resistant to digestion may find unique applications. Starch hydrolyzing enzymes display essential roles in the production of resistant starch, and the most important is pullulanase. In current study, an industrially important pullulanase from Bacillus licheniformis has been produced and purified for its potential use in synthesis of resistant starch type III. B. licheniformis is an efficient producer of pullulanase when checked on pullulan containing media. Different organic substrates were evaluated for production of pullulanase in fermentation media and ascribe that corn bran was an excellent source for pullulanase production (16 U/mg). The pullulanase was purified to homogeneity with a molecular mass of about 75 kDa on SDS-PAGE and specific activity of 191 U/mg. Pullulanase was optimally active at pH 5 and 50°C. In addition, Ca+2 and EDTA have no noticeable effect on pullulanase activity. Substrate specificity and thin layer chromatography results specify pullulanase as pullulanase type I. Purified pullulanase were used for the preparation of resistant starch from maize flour. Addition of pullulanase into gelatinized maize starches significantly increased the yield of resistant starch III. Final yields of resistant starch III with pullulanase treated maize starches were increased (14.32%) as compare to untreated (1.34%) and heat treated maize starch (4.25%). These results provided new information that may shed light on the further development of method to modify starches with enzymatic treatment.
Publication Details
Page(s) 699-709
DOI 10.21162/PAKJAS/21.9847
Published Journal: Pakistan Journal of Agricultural Sciences, Volume: 58, Issue: 2, Year: 2021
Keywords
Bacillus licheniformis pullulanase organic substrate corn bran resistant starch
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