Rdr1active against a fungal disease, black spot, of roses. Rdr1 locus consists of nine genes named as muRdr1A to muRdr1I. Functional and molecular characterization of this locus, revealed the presence of one gene, muRdr1H, active against Dort E4, race 6 of Diplocarpon rosae. In the present study we aimed to analyze the protein sequences of three members of this Rdr1 gene family for their homologies, important domains, conserved regions and selection pressure to get insights of their functionality based on protein sequence. The protein sequences of one active gene, muRdr1H and two inactive genes, muRdr1C and muRdr1G, were deduced and dissected using internet based bioinformatics tools. All three proteins belong to the TIR-NBSLRR family of resistance genes. Although these proteins carry all necessary domains and conserved regions necessary for the functionality of TIR-NBS-LRR proteins, LRR region of the proteins, found under selection.